201 studies found
mRNA samples were collected from circulating CD4+ and CD8+ T-cells from healthy donors as well as donors with severe and non-severe asthma.
NHEK were treated with IL-19, IL20, IL-22, and IL24, with controls untreated, along with IL1b, IFN gamma, and KGF.
Interim analysis. Study outline is available in Diabetologia (2014) 57:1132–1142.
Twelve whole-blood DNA samples from six SLE patients and six controls were analyzed by Illumina HumanMethylation27 BeadChip.
The series contain eight UC samples with macroscopic signs of inflammation, 13 UC smaples without macroscopic signs of inflammation, five control subjects.
The genome-wide study used matched case-control design, with 97 samples of 90 patients in three disease groups. Matched control samples were used and several (7) technical replicates were performed.
Fibroblast-like cells (FLS) were purified from synovial biopsies from RA patients. Briefly, minced synovial fragments were digested in 1 mg/ml hyaluronidase solution (Sigma Aldrich, St Louis, MO) for 15 minutes at 37°C and 6 mg/ml collagenase type IV (Invitrogen, Paisley, UK) for 2 hours at 37°C. Next, cells were washed, resuspended in high-glucose Dulbecco’s Modified Eagle Medium (Invitrogen) supplemented with 1% antibiotics-antimycotics (Invitrogen) and 1% MEM sodium pyruvate (Invitrogen), ...
We have recruited 9 RA patients starting immunotherapy with rituximab. The same day of the first infusion we obtained the three different cell type samples: whole blood (preserved in PaxGene tubes), B cells (using negative selection) and CD4+ T cells (using negative selection). At week 24 of treatment we determined the clinical response using the RelDas score. All tissue samples were processed in parallel (i.e. RNA extraction and microarray profiling with Illumina WG6 Beadchip) in order to mi...