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235 studies found
Key Regulatory Molecules of Cartilage Destruction in...
To reveal the RA-related chondrocyte gene expression profile, genome-wide microarray analysis of RASF stimulated human chondrocytes compared to NDSF stimulation was performed. Unstimulated chondrocytes were analyzed for baseline gene expression. For microarray analysis of RASF and NDSF stimulated and unstimulated chondrocytes, respectively, two RNA pools were analyzed, each pool consisting of equal amounts of RNA from three different donors.
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Lung biopsies from asthmatics pre- and post- steroid...
Endobronchial biopsies from 27 asthmatics and 13 healthy controls were assayed on expression microarrays. Biopsies were obtained from asthmatics before and one week after treatment with Flovent, an inhaled corticosteroid commonly used to treat asthma.
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Macrophages heterogeneity in human atherosclerotic plaques
18 RNG/MRC two-colour oligonucleotide microarrays (Le Brigand et al. 2006) were used to generate global mRNA expression profiles for GM-CSF-induced, M-CSF-induced, and peritoneal macrophages. The microarray experiments were conducted either as a common reference (peritoneal-like macrophages) design or using a direct design by hybridizing Granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced human monocyte-derived macrophage (GM-MØ) and CSF-induced human monocyte-derived macrophage...
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Mapping of disease-associated expression polymorphi...
RNA was obtained from peripheral blood CD4+ lymphocytes at four study centers. Peripheral blood (17 cc) was collected into BD Vacutainer CPT tubes (BD Diagnostics, Franklin Lakes, New Jersey) and placed on ice. Samples were centrifuged within 1 hour of collection for 20 minutes at 1700RCF, followed by mononuclear cell layer isolation and suspension in 10 ml of PBS. We isolated CD4+ lymphocytes using anti-CD4+ microbeads by column separation (Miltenyi Biotec, Auburn, CA) using 20 µl anti-CD4+ ...
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Microarray predictor of response to infliximab in rh...
Blood RNA from 48 Rheumatoid Arthritis patients was extracted at baseline of anti-TNF alpha treatment with infliximab. From these 44 reached week 14 of treatment at which the clinical response was determined. Using 2/3 of the sample we built a multigene predictor using kNearest-Neighbour algorithm and Leave-One Out cross validation. The remaining 1/3 of the sample was used to estimate the precision accuracy of the predictor model.
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Molecular consequences of Csf1R (M-CSF receptor) inh...
RNA was extracted from chronic epilepsy mouse hippocampi after 2 weeks daily exposure to vehicle, PLX3397 3mg/kg or 30 mg/kg. Healthy, non-epileptic mice, were added separately to determine baseline expression.
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