Mapping of disease-associated expression polymorphisms in primary peripheral blood CD4+ lymphocytes
RNA was obtained from peripheral blood CD4+ lymphocytes at four study centers. Peripheral blood (17 cc) was collected into BD Vacutainer CPT tubes (BD Diagnostics, Franklin Lakes, New Jersey) and placed on ice. Samples were centrifuged within 1 hour of collection for 20 minutes at 1700RCF, followed by mononuclear cell layer isolation and suspension in 10 ml of PBS. We isolated CD4+ lymphocytes using anti-CD4+ microbeads by column separation (Miltenyi Biotec, Auburn, CA) using 20 µl anti-CD4+ Micro beads per 106 total cells
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- Primary Purpose
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- Description of Cohorts
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- Informed Consent Given?
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- Multi-center Study?
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- Organisms
- Homo sapiens
- Number of subjects
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- Diseases
- asthma
- Sample Sources
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- Type of Samples Collected
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