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208 studies found

ZNF750 in late keratinocyte differentiation

Gene expression analysis: To determine the differentaition signature for HaCaT keratinocytes, with ZNF750 gene silencing, total RNA was isolated in biologic triplicates from cells induced to differentiate for twelve days and hybridized to Affymerix Human Gene 1.0 ST arrays.



Isolated fresh bronchial epithelial cells were placed into trizol for further analysis.


chronic temporal lobe epilepsy: biopsy hippocampus

129 human hippocampus samples



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Preclinical toxicological assessment in animal models


expression profiling of responsiveness to infliximab...

The PBMCs were isolated from venous blood by Ficoll-Hypaque and total RNAs were extracted, measured at 260 nm and frozen at -80°C until further used. An internal, arbitrary standard was made of a mixture of total RNAs from PBMCs taken from 3 healthy donors. The oligodT-primed poly(A) mRNAs were labelled with [α33P]dCTP as previously described [Coulouarn et al 2004], and the resulting, labelled cDNAs were immediately used for hybridization. Our array covering 12,000 cDNA probes for 10,000 non-...


expression profiling of rheumatoid arthritis patient...

In total 42 patients were treated with anti-TNF. RNA was isoloated from white blood cells and anti-TNF responders (n=18) were compared to nonresponders (n=24) regarding expression profiles


whole brains from nicotinic acetylcholine receptor s...

The experimental groups included six wild-type mice and five beta4-/- mice. All mice were 6-9 weeks old. Total RNA was extracted from whole brain tissue of the mice.