Data Catalog

The Nasal Methylome and Childhood Atopic Asthma

Case control design with nasal epithelial cells from 36 atopic asthmatic and 36 nonatopic nonasthmatic children from the inner cityNote: The Sample data tables are incomplete (truncated at 65535 rows, related to row-limit in older version of Excel).

201

The peroxisome proliferator-activated receptor-gamma...

Human PBMCs were isolated; RNA from healthy and lupus PBMCs was extracted and processed for hybridization on Affymetrix microarrays.Samples are paired as follows: 1-2, 3-4, 5-6, 7-8, 9-10, 11-12, 13-14, 15-16, 19-20.

202

The relationship between the gene expression profile...

All patients were hybridized in a dual channel reference design where the reference was always labeled with cy3 and the samples with cy5. In total, RNA was extracted from synovial biopsies of 62 patients. 18 patients were good responding, 30 moderate responding and 14 non-responding. Universal Human Reference RNA from Stratagene was used as reference. All samples were amplified with the RiboAmp II kit before labeling and hybridization.

203

The type of phagocytic receptor modulates gene expre...

All samples and controls were carried out in triplicates. J774.A1 cells (mouse macrophage-like cell line) were seeded onto 6-well plates one day before the experiment. Subsequently, cells were incubated with serum-free DME medium containing 0.01 % latex beads of 1 µm diameter coupled to the following ligands: avidin (Av), the Fc fragment of mouse IgG (Fc), lipopolysaccharides from Klebsiella pneumoniae (LPS) and mannan from Saccharomyces cerevisae (Man) for 30 and 60 minutes. After incubation...

204

Time course microarray data following GR activation ...

MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs. Total RNA from each sample was extracted using Qiagen’s RNeasy Kit. Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility. HG-U133A genechip contains 22,215 probe sets that represent approximatel...

205

Time course of bone marrow-derived macrophages simul...

Bone marrow-derived macrophages stimulated with LPS for 0, 20, 40, 60, 80, 120, 240 and 360 minutes.

206

Time-dependent transcriptomic profiles of mouse hipp...

40 animals were in the study; 80 samples were analyzed via Illumina beadchip microarray, a total which included 2 samples (IL and CL hippocampus) from each animal. Of the 80 samples,the n were as follows: IL-KA-7d, 8; CL-KA-7d, 8; IL-KA-28d, 9; CL-KA-28d, 9; IL-KA-60d, 9; CL-KA-60d, 9; IL-sham-7d, 4; CL-sham-7d, 4; IL-sham-28d, 5; CL-sham-28d, 5; IL-KA-60d, 5; CL-KA-60d, 5.

207