208 studies found
All samples and controls were carried out in triplicates. J774.A1 cells (mouse macrophage-like cell line) were seeded onto 6-well plates one day before the experiment. Subsequently, cells were incubated with serum-free DME medium containing 0.01 % latex beads of 1 µm diameter coupled to the following ligands: avidin (Av), the Fc fragment of mouse IgG (Fc), lipopolysaccharides from Klebsiella pneumoniae (LPS) and mannan from Saccharomyces cerevisae (Man) for 30 and 60 minutes. After incubation...
MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs. Total RNA from each sample was extracted using Qiagen’s RNeasy Kit. Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility. HG-U133A genechip contains 22,215 probe sets that represent approximatel...
Bone marrow-derived macrophages stimulated with LPS for 0, 20, 40, 60, 80, 120, 240 and 360 minutes.
40 animals were in the study; 80 samples were analyzed via Illumina beadchip microarray, a total which included 2 samples (IL and CL hippocampus) from each animal. Of the 80 samples,the n were as follows: IL-KA-7d, 8; CL-KA-7d, 8; IL-KA-28d, 9; CL-KA-28d, 9; IL-KA-60d, 9; CL-KA-60d, 9; IL-sham-7d, 4; CL-sham-7d, 4; IL-sham-28d, 5; CL-sham-28d, 5; IL-KA-60d, 5; CL-KA-60d, 5.
Total 24 AERD (Aspirin-exacerbated respiratory disease) and 18 ATA(aspirin-tolerant asthma) samples were used to find bio-markers using 35K op-array.
Colonic biopsies were obtained from forty-five patients with UC (20 left-sided colitis, 19 pancolitis, and 6 UC-associated dysplasia) and 15 controls. The Affymetrix GeneChip Human Genome U133 Plus 2.0 was used
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