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208 studies found

The type of phagocytic receptor modulates gene expre...

All samples and controls were carried out in triplicates. J774.A1 cells (mouse macrophage-like cell line) were seeded onto 6-well plates one day before the experiment. Subsequently, cells were incubated with serum-free DME medium containing 0.01 % latex beads of 1 µm diameter coupled to the following ligands: avidin (Av), the Fc fragment of mouse IgG (Fc), lipopolysaccharides from Klebsiella pneumoniae (LPS) and mannan from Saccharomyces cerevisae (Man) for 30 and 60 minutes. After incubation...

177

Time course microarray data following GR activation ...

MCF10A-Myc cells were subjected to 72 hrs of growth factor withdrawal followed by treatment with either vehicle (ethanol) or Dex (10-6 M) for 0.5, 2, 4 or 24 hrs. Total RNA from each sample was extracted using Qiagen’s RNeasy Kit. Preparation of biotinylated cRNA and hybridization to oligonucleotide arrays (Affymetrix human genome genechip HG-U133A) were performed at the University of Chicago Microarray Core Facility. HG-U133A genechip contains 22,215 probe sets that represent approximatel...

178

Time course of bone marrow-derived macrophages simul...

Bone marrow-derived macrophages stimulated with LPS for 0, 20, 40, 60, 80, 120, 240 and 360 minutes.

179

Time-dependent transcriptomic profiles of mouse hipp...

40 animals were in the study; 80 samples were analyzed via Illumina beadchip microarray, a total which included 2 samples (IL and CL hippocampus) from each animal. Of the 80 samples,the n were as follows: IL-KA-7d, 8; CL-KA-7d, 8; IL-KA-28d, 9; CL-KA-28d, 9; IL-KA-60d, 9; CL-KA-60d, 9; IL-sham-7d, 4; CL-sham-7d, 4; IL-sham-28d, 5; CL-sham-28d, 5; IL-KA-60d, 5; CL-KA-60d, 5.

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To identify gene markers which differentiate between...

Total 24 AERD (Aspirin-exacerbated respiratory disease) and 18 ATA(aspirin-tolerant asthma) samples were used to find bio-markers using 35K op-array.

181

Transcriptional analysis of left-sided colitis, panc...

Colonic biopsies were obtained from forty-five patients with UC (20 left-sided colitis, 19 pancolitis, and 6 UC-associated dysplasia) and 15 controls. The Affymetrix GeneChip Human Genome U133 Plus 2.0 was used

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