Data Catalog

ImmGen Microarray Phase 1

This Series record provides access to Immunological Genome Project data submitted to GEO.

129

Immunity and Defense Genes in Peripheral Blood Monon...

33 samples analyzed corresponding to 18 Rheumatoid Arthritis Patients and 15 Controls

130

Induction of Dendritic Cell-like Phenotype in Macrop...

Human blood was drawn from the antecubital veins of healthy blood donors and provided as buffy coats by the Virginia Blood Services (Richmond, VA). The mononuclear fractions were pooled from four unidentified donors to decrease individual variations in monocytes. Mixed peripheral blood mononuclear cells (PBMCs) were isolated by Histopaque 1.077 (Sigma Diagnostics, Inc., St. Louis, MO). Following centrifugation, the mononuclear layer was removed and washed with PBS containing 0.02% ethylenedia...

132

Inflammatory Expression Profiles in Monocyte to Macr...

Monocytes were obtained from 20 patients with SLE and 16 healthy controls and were in vitro differentiated into macrophages. Subjects also underwent laboratory and imaging studies of the coronary arteries, carotid arteries, and aorta to evaluate for subclinical atherosclerosis.

133

Integrated Network and Microarray Analysis to Identi...

The 32 patients of this study were divided in 2 groups corresponding to the extreme quartiles of FE values. The first group are the patients with low Ejection fraction and the second group are the patients with high ejection fraction.Blood sample: 2.5 mL harvested in PAXgene® Blood RNA tubes (PreAnalytix)RNA extraction: PAXgene® Blood RNA kit (Qiagen)We used a Universel Reference RNA (Stratagene)RNA amplification and labelling: kit Amino Allyl MessageAmp II (Ambion)We hybridized 4 microarray...

134

Interactions of cytokines in peripheral blood cells ...

Peripheral blood was obtained from patients with SLE (n=11) and healthy women (n=6). Gene expression profile was analyzed using DNA microarray covering 30,000 human genes. Differentially expressed immune response-related genes were selected and analyzed by using Expression Analysis Systemic Explorer (EASE) based on Gene Ontology (GO) followed by network pathway analysis with Ingenuity Pathways Analysis (IPA).

135