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24 studies found
Expression data from human bronchial airway smooth m...
To evaluate IL-17A-inducible gene transcripts, primary human bronchial ASM cells from 3 mild asthmatic and 3 healthy donors were treated for 2h with IL-17 [10ng/ml] and were probed with the Affymetrix GeneChip array. The 2h time point was carefully chosen in order to identify primary response gene targets and to avoid confounding autocrine mechanisms mediating indirect, or late-phase gene expression responses. Non-stimulated ASM cells from the same patients were used as controls.
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Expression data from peripheral blood - blood draws...
Blood was collected immediately prior to, and two hours after challengeThe change in gene expression (post expression minus pre expression) in ERs was compared with the change in gene expression in DRs using age and sex as covariatesPreprocessing and probeset filtering were applied to the entire dataset (28 CEL files) using the Factor Analysis for Robust MicroArray Summarization (farms) package in RThe Linear Models for MicroArrays (limma) package was used to determine differential gene expre...
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Gene expression changes in blood RNA after swimming ...
Expression profile differences were determined between total RNA extracted before and after exposure to trihalomethanes present in swimming pool water for 40 minute from whole blood samples from 33 healthy human individuals.
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Genome-Wide Profiling of Airway Epithelial Cells in ...
1) Comparison of gene expression in asthmatics to healthy controls2) Comparison of smokers to healthy controls3) Comparison of change in gene expression in the fluticasone treated group to the change in the placebo treated group
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Human rhinovirus infection causes different DNA meth...
Bisulphite converted DNA from matched mock and human rhinovirus-16 infected nasal epithelial cells from Healthy (n=3) and Asthmatics (n=6) adults were hybridized to the Illumina DNA methylation 450K Bead Array.
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Lung biopsies from asthmatics pre- and post- steroid...
Endobronchial biopsies from 27 asthmatics and 13 healthy controls were assayed on expression microarrays. Biopsies were obtained from asthmatics before and one week after treatment with Flovent, an inhaled corticosteroid commonly used to treat asthma.
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Mapping of disease-associated expression polymorphi...
RNA was obtained from peripheral blood CD4+ lymphocytes at four study centers. Peripheral blood (17 cc) was collected into BD Vacutainer CPT tubes (BD Diagnostics, Franklin Lakes, New Jersey) and placed on ice. Samples were centrifuged within 1 hour of collection for 20 minutes at 1700RCF, followed by mononuclear cell layer isolation and suspension in 10 ml of PBS. We isolated CD4+ lymphocytes using anti-CD4+ microbeads by column separation (Miltenyi Biotec, Auburn, CA) using 20 µl anti-CD4+ ...
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Nasal scrape gene expression profiling in asthmatics
Nasal scrape samples were collected from asthmatics and healthy controls and subjected to expression profiling using Affymetrix HG-U133Plus2.0 microarrays.
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