Search studies
24 studies found
Microarray predictor of response to infliximab in rh...
Blood RNA from 48 Rheumatoid Arthritis patients was extracted at baseline of anti-TNF alpha treatment with infliximab. From these 44 reached week 14 of treatment at which the clinical response was determined. Using 2/3 of the sample we built a multigene predictor using kNearest-Neighbour algorithm and Leave-One Out cross validation. The remaining 1/3 of the sample was used to estimate the precision accuracy of the predictor model.
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Mutual Antagonistic Relationship Between Prostagland...
For details, see Mathieu MC et al, EJI, issue 7, 2008
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The relationship between the gene expression profile...
All patients were hybridized in a dual channel reference design where the reference was always labeled with cy3 and the samples with cy5. In total, RNA was extracted from synovial biopsies of 62 patients. 18 patients were good responding, 30 moderate responding and 14 non-responding. Universal Human Reference RNA from Stratagene was used as reference. All samples were amplified with the RiboAmp II kit before labeling and hybridization.
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Transcriptional response to hypoxia of normal and rh...
Synovial fibroblasts obtained from 6 patients with rheumatoid arthritis (RASF) and 6 sex and age matched adult healthy donors (HSF) were used. SF cultures were incubated for 22 hours under normoxic or hypoxic (0.5% O2) conditions. Nine experiments per group were performed, single experiments with three SF lines, and duplicated in other three lines per group. All 18 normoxia-hypoxia experiments (36 microarray data) were used for paired analysis of the changes induced by hypoxia in HSF or RASF.
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Whole blood transcript profiling of rheumatoid arthr...
Patients' response to anti-TNF was assessed using EULAR score and patients were classified as responders, moderate responders and non-responders. Genes correlating with the response status have been identified.
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expression profiling of responsiveness to infliximab...
The PBMCs were isolated from venous blood by Ficoll-Hypaque and total RNAs were extracted, measured at 260 nm and frozen at -80°C until further used. An internal, arbitrary standard was made of a mixture of total RNAs from PBMCs taken from 3 healthy donors. The oligodT-primed poly(A) mRNAs were labelled with [α33P]dCTP as previously described [Coulouarn et al 2004], and the resulting, labelled cDNAs were immediately used for hybridization. Our array covering 12,000 cDNA probes for 10,000 non-...
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expression profiling of rheumatoid arthritis patient...
In total 42 patients were treated with anti-TNF. RNA was isoloated from white blood cells and anti-TNF responders (n=18) were compared to nonresponders (n=24) regarding expression profiles
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