Data Catalog

Type I Interferon: Potential Therapeutic Target for ...

A total of 82 Affymetrix HGU133 Plus arrays were run on 54 total subjects. This includes: skin biopsy samples from 21 normal healthy donors (Normal-1 to Normal-21), 56 skin biopsy samples from 28 psoriasis patients who had match lesional and uninvolved (non-lesional) tissue (NS-1/LS-1 to NS-9/LS-9, NS-11/LS-11 to NS-14/LS-14, and NS-16/LS-16 to NS-30/LS-30), and 5 samples from psoriasis patients that only provided lesional skin biopsies (LS-10, LS-15, and LS-31 to LS-33).

IL-17A is an essential cytokine to sustain pathogeni...

In a phase 1, randomized, double-blind, placebo-controlled dose escalation trial, patients with chronic psoriasis were randomized to receive 3 doses of subcutaneous LY2439821 at 5 mg (n=8), 15 mg (n=8), 50 mg (n=8), 150 mg (n=8) or placebo (n=8) at weeks 0, 2 and 4. Repeat biopsies were taken from the same lesional area at baseline, week 2 and 6. At week 6, a PASI75 was observed in 0/8, 2/8, 5/7 and 8/8 patients receiving 5 mg, 15 mg, 50 mg or 150 mg LY2439821 respectively and 0/8 patients re...

Post-therapeutic relapse of psoriasis associated wit...

To determine the transcsriptome of skin samples in 4 responding patients who relapsed after receiving efalizumab for treatment of psoriasis, using paired baseline non-lesional (=NL, n=2); lesional (LS, n=4);week 12 post-treatment (week 12, n=4), and relapse (n=4).

Human keratinocytes have a response to injury that u...

Compare keratinocyte response to cell disassociation with (1) epidermal samples isolated by laser caputre microscopy and (2) cultured KCs

Expression data from skin biopsy samples from patien...

Skin biopsy samples (n=170) were collected at baseline for RNA extraction and microarray analysis from 85 patients with moderate-to-severe psoriasis without receiving active psoriasis therapy. The sample title is concatenated as <sample collection site ID>_<subject ID>_<skin type>.

Expression data from primary human keratinocytes exp...

Primary keratinocytes from three donors (subjects 1, 2, and 3) were obtained and were either untreated (control) or exposed to cytokines (IL-4, IL-13, IFN-alpha, IFN-gamma and TNF). For the IL17A samples, primary keratinocytes were obtained from six donors, with cells derived from three donors treated with IL-17A and cells derived from the other three donors left untreated (i.e., unpaired control samples).

Gene expression profiles in synovial biopsies from p...

GeneChip® Human Genome U133 Plus 2.0 Arrays (Affymetrix UK Ltd., High Wycombe, UK) were hybridized in monoplicates with 10 μg biotinylated cRNA, using synovial biopsy samples obtained in 4 patients with SA (based on the presence of psoriasis or x-ray evidence of sacro-iliacal joint involvement), 5 patients with MIC, based on the identification of urate or calcium pyrophosphate crystals in the synovial fluid, 7 patients with RA (ACR 1987 criteria), 4 patients with SLE (ACR criteria) and 5 pati...

ZNF750 in late keratinocyte differentiation

Gene expression analysis: To determine the differentaition signature for HaCaT keratinocytes, with ZNF750 gene silencing, total RNA was isolated in biologic triplicates from cells induced to differentiate for twelve days and hybridized to Affymerix Human Gene 1.0 ST arrays.