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19 projects found
Human immune cell transcriptome
In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. This comprehensive dataset enabled us to not only establish which genes were expressed in each leukocyte type, but also which genes were expressed in each subset after activation. The used of a comprehensive dataset of gene profiles from all the major human leukocyte subsets enabled a novel and powerful means for identification of genes associated...
Keywords: Cell type comparison macrophages expression profiling by array
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ImmGen Microarray Phase 1
Gene-expression microarray datasets generated as part of the Immunological Genome Project (ImmGen). Primary cells from multiple immune lineages are isolated ex-vivo, primarily from young adult B6 male mice, and double-sorted to >99% purity. RNA is extracted from cells in a centralized manner, amplified and hybridized to Affymetrix 1.0 ST MuGene arrays. Protocols are rigorously standardized for all sorting and RNA preparation. Data is released monthly in batches of cell populations.
Keywords: NCBI GEO macrophages expression profiling by array
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Induction of Dendritic Cell-like Phenotype in Macrop...
Foam cell formation from monocyte-derived macrophages is a hallmark of atheroscle-rotic lesions. Aspects of this process can be recapitulated in vitro by exposing MCSF-induced or platelet factor4 (CXCL4)-induced macrophages to oxidized (ox) or minimally modified (mm) low density lipoprotein (LDL). We measured gene expression in periph-eral blood mononuclear cells (PBMCs), monocytes and macrophages treated with CXCL1 (GRO-α) or CCL2 (MCP-1) as well as foam cells induced by native LDL, mmLDL or...
Keywords: PBMC monocytes macrophages foam cells chemokines macrophages expression profiling by array
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Macrophages heterogeneity in human atherosclerotic plaques
This study aims to characterize the transcriptional profile of Granulocyte-macrophage colony-stimulating factor induced macrophages (GM-MØ) and M-CSF macrophages (M-MØ) and to investigate in situ a subset of genes and their products specific to each phenotype in human atherosclerosis plaques
Keywords: NCBI GEO macrophages expression profiling by array
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Mechanisms Establishing TLR4-Responsive Activation S...
This SuperSeries is composed of the SubSeries listed below.
Keywords: NCBI GEO macrophages expression profiling by array genome binding/occupancy profiling by high throughput sequencing expression profiling by high throughput sequencing
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Monocytes of patients with familial hypercholesterol...
Background: Elevated plasma cholesterol promotes the formation of atherosclerotic lesions in which monocyte-derived lipid-laden macrophages are frequently found. To analyze, if circulating monocytes already show increased lipid content and differences in lipoprotein metabolism, we compared monocytes from patients with Familial Hypercholesterolemia (FH) with those from healthy individuals.Methods: Cholesterol and oxidized cholesterol metabolite serum levels of FH and of healthy, gender/age mat...
Keywords: Micro-array analysis cell type comparison, macrophages expression profiling by array
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Peritoneal and testicular macrophage mrna expression...
Ascending bacterial infections of the male genitourinary tract by Escherichia coli are an important cause of male infertility. Thus understanding mechanisms by which immunocompetent cells such as testicular macrophages (TM) respond to infection and how bacterial pathogens manipulate defense pathways is of great relevance. Following infection with uropathogenic E. coli (UPEC) whole genome expression profiling revealed induction of the calcium-dependent nuclear factor of activated T cells (NFAT...
Keywords: NCBI GEO macrophages expression profiling by array
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Similarities and differences in the transcriptome of...
Phenotypic and functional diversity between macrophage subpopulations reflects their plasticity to respond to microenvironmental signals. Apart from detecting differences in expression profiles, the comparison of the transcriptomes of different macrophage populations may also allow the definition of molecular similarities between these subsets.Transcriptome analysis of human Kuppfer cells, alveolar, splenic and atherosclerotic plaque residing macrophages using microarrays, identified 42 genes...
Keywords: NCBI GEO macrophages expression profiling by array
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