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142 projects found

Colon biopsies from Crohns patients and healthy controls

The aim of this study was to investigate differential intestinal gene expression in patients with Crohn's disease (CD) and controls. 172 biopsies from CD and control subjects were studied. Endoscopic biopsies were taken at ileocolonoscopy from five specific anatomical locations including the terminal ileum for RNA extraction.

Keywords: NCBI GEO expression profiling by array


Combination of peripheral blood gene expression prof...

We used microarrays to identify markers predicting responder status in tocilizumab treatment in rheumatoid arthritis in 13 patients at week 0 and week 4 of treatment.

Keywords: NCBI GEO expression profiling by array


Comparison of human Rheumatoid arthritis patient per...

Leukocytapheresis((LCAP) is a safe, unique therapy prevalent for intractable rheumatoid arthritis even in case of drug allergy or infectious state. To investigate how to improve LCAP efficacy, we have conducted genes expressions analysis from peripheral blood of Rheumatoid arthritis patients using high resolution DNA microarray just before and after LCAP procedure.

Keywords: NCBI GEO expression profiling by array


Comparison of mRNA expression in circulating T-cells...

Comparison of mRNA expression showed widespread changes in the circulating CD8+ but not CD4+ T-cells from patients with severe asthma. No changes were observed in the CD4+ and CD8+ T-cells in non-severe asthmatics versus healthy controls. Bioinformatics analysis showed that the changes in CD8+ T-cell mRNA expression were associated with multiple pathways involved in T-cell activation. As with mRNAs, we also observed widespread changes in expression of non-coding RNA species including natural ...

Keywords: NCBI GEO expression profiling by array


DNA methylation in systemic lupus erythematosus patients

To screen specific DNA methylation markers in systemic lupus erythematosus (SLE) patient's blood DNA, whole-blood DNAs from 6 female SLE patients and 6 female controls were analyzed by methylation microarray.

Keywords: NCBI GEO methylation profiling by array


Determinants of excess genetic risk of acute myocard...

Peripheral blood samples of patients with acute myocardial infarction were matched with those of control patients to identify possible differences in corresponding gene expression profiles. The controls were matched to cases based on gender, age, status of diabetes mellitus and smoking status. Six months cardiovascular survival status of the cases was used to identify two distinct subgroups among the cases. Linear models for microarray data (‘limma’) were employed to identify differential gen...

Keywords: NCBI GEO expression profiling by array


Differential effects of TNFalpha and IL1beta on FLS ...

TNFalpha and IL1beta play a pathogenic role in rheumatoid arthritis. Both cytokines are known to activate cytokine and metalloproteinase secretion by synovial fibroblasts. In the present study, we wanted to investigate whether TNFalpha and IL1beta displayed differential effects on cultured Fibroblast-like Synovial Cells derived from RA patients. Global gene expression analyses indicated that both cytokines induced similar genes in these cells.

Keywords: NCBI GEO expression profiling by array


Differential expression of rituximab responders vs. ...

New and effective therapeutical options are available for the treatment of Rheumatoid Arthritis. One of such treatments is rituximab, and chimeric anti-CD20 antibody that selectively depletes the CD20+ B cell subpopulation.Similar to established anti-TNF alpha therapies, there is a subgroup of RA patients that do not experience significant clinical response. Therefore, one of the major necessities in actual RA therapeutical management is to identify reliable predictors of the response to thi...

Keywords: NCBI GEO expression profiling by array