Search projects
185 projects found
IL-1 treatment of human epidermal keratinocytes
Interleukin-1 is a proinflammatory and immunomodulatory cytokine that plays a crucial role in inflammatory diseases of the skin, including bacterial infections, bullous diseases, UV damage and especially psoriasis. To characterize the molecular effects of IL-1 in epidermis, we defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1a. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal t...
Keywords: Time course expression profiling by array
177
Human colon expression in healthy, CD, treated CD, and UC
Activation of inflammatory pathways in human IBDIL-6:STAT3 pathways are activated in the affected colon in IBD. However, the functional implications of this are not known. We hypothesized that pro-inflammatory IL-6:STAT3 dependent networks would be up regulated in the colon of pediatric patients with Crohn Disease (CD) and Ulcerative Colitis (UC), and that these would regulate leukocyte survival, proliferation, and recruitment to the gut. These experiments tested differential colon gene ex...
Keywords: Single time point in CD UC and healthy controls. expression profiling by array
178
Human Chronic Obstructive Pulmonary Disorder (COPD) ...
Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disease with complex pathological features and largely unknown etiologies. Identification and validation of biomarkers for this disease could facilitate earlier diagnosis, appreciation of disease subtypes and/or determination of response to therapeutic intervention. To identify gene expression markers for COPD, we performed genome-wide expression profiling of lung tissue from 56 subjects using the Affymetrix U133 Plus 2.0 a...
Keywords: Disease state marker expression profiling by array
179
Hippocampal gene expression signature following infl...
We used a systemic inflammatory challenge in animals with chronic mesial temporal lobe epilepsy to identify novel molecular pathways involved in seizure regulation. Mice were first rendered epileptic by a single intrahippocampal injection of kainic acid (KA). After a period of two-three weeks, mice developed spontaneous seizures and then received a single intraperitoneal injection of lipopolysaccharide (LPS) to mimic systemic inflammation, or saline as a control. Gene expression analysis by m...
Keywords: NCBI GEO expression profiling by array
180
Expression data of synovial cells isolated from pati...
Rheumatoid arthritis (RA), a chronic and systemic disease of unknown etiology, is characterized by hyperplasia of synovial cells, which ultimately lead to the destruction of cartilage and bone. To elucidate the molecular mechanisms that lead to RA, we analyzed synovial cells established from patient with RA by oligonucleotide microarrays. Gene expression profiles reveal a novel pathophysiologic function of RA synovial cells as a generator of oxidative stress, and a self-defense mechanism ag...
Keywords: cell type comparison disease state analysis expression profiling by array
181
Whole genome transcription and DNA methylation analy...
Our study has demonstrated that significant number of differential genes in SLE was involved in IFN, TLR signaling pathways and inflammatory cytokines. The enrichment of differential genes has been associated with aberrant DNA methylation, which may be relevant to the pathogenesis of SLE. Our observations laid the groundwork for further diagnostic and mechanistic studies of SLE and LN.
Keywords: NCBI GEO methylation profiling by genome tiling array
182
Expression data from mouse bone marrow macrophages f...
Bone marrow macrophages were cultured from 16 week old apoE-deficient F2 mice from an AKRxDBA/2 intercrossGene expression profiling was performed using Affy 430v2 arrays, and 1967 informative SNP markers were genotyped for each mouseSupplementary file of SNP data attached below from ParAllele 5K mouse linkage panel
Keywords: Genetic-genomic macrophages expression profiling by array
183
Blockade of interferon-γ normalizes IFN regulated ge...
Normal donor blood was incubated with or without IFN-g stimulation to establish an IFN-g gene signature. Systemic lupus erythematosus subjects were treated with placebo or AMG 811, a therapeutic anti-IFN--g antibody, and changes in the IFN--g signature in whole blood of these subjects was measured.
Keywords: NCBI GEO expression profiling by array
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