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185 projects found
Early and long-standing rheumatoid arthritis: molecu...
At a given time, rheumatoid arthritis (RA) is a heterogeneous disease and its underlying molecular mechanisms is still poorly understood. Because previous microarrays studies have only focused on late RA stages, we aimed to compare the biological and molecular profiles in early and long-standing (LS) RA. Synovial biopsies obtained by arthroscopy were performed in 4 early untreated RA patients, 4 LS treated RA patients and 7 control patients. Extracted mRNA were linearly amplified and used fo...
Keywords: NCBI GEO expression profiling by array
97
Activation of mTOR controls the loss of TCRζ in lupu...
CD3-positive T cells were negatively isolated from 10 SLE patients and 9 healthy controls without SLE. All of the SLE samples and control samples were compared with one another to identify baseline differences in expression due to the disease. Next, T cell preparations from 4 of the control subjects were stimulated with either Nitric Oxide (NOC-18) 600 uM for 24hr or stimulated through CD3/CD28 for 24hr to determine which genes were responsive to these signaling mechanisms. Here, we show th...
Keywords: NCBI GEO expression profiling by array
98
Interactions of cytokines in peripheral blood cells ...
SLE patients are always with various disease manifestation. Various cytokines are pointed interacting and playing pathological roles in SLE although the etiopathology is still obscure. In this study, we aimed to investigate the effects of cytokine interactions in the immune response of SLE patients. Overexpressed interferon-inducible(IFI) genes were confirmed in peripheral blood from SLE patients. Using network-based analysis on the immune response-related genes, several networks including cy...
Keywords: NCBI GEO expression profiling by array
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Gene Expression Analysis in Peripheral Blood of Pati...
Peripheral blood cells in patients with rheumatoid arthritis before/after IL-6 inhibitionThe obtained conclusions on the change of each gene expression level before and after MRA medication were as follows.(1) In the case of IL-6, the specific time profile that the expression level dropped at day 21 and increased to some extent at day 42 was observed in 2 of 18 subjects. In other subjects, quite small change or small increase after day 7 was observed.(2) In the case of IL-6R as well as in IL-...
Keywords: NCBI GEO expression profiling by array
100
Gene Expression Profiling of Rat Hippocampus Followi...
Soman (O-Pinacolyl methylphosphonofluoridate) is a potent neurotoxicant. Acute exposure to soman causes profound inhibition of the critical enzyme acetylcholinesterase, resulting in excessive levels of the neurotransmitter acetylcholine. Excessive acetylcholine levels cause convulsions, seizures, and respiratory distress. The initial cholinergic crisis can be overcome by rapid anti-cholinergic therapeutic intervention, resulting in increased survival. However, conventional treatments do n...
Keywords: Time-course toxicant exposure expression profiling by array
101
Transcriptional Response of Human Microglial Cells t...
Microglial cells are resident macrophages in the central nervous system (CNS) and play a pivotal role in the innate and adaptive immune responses against microbial infections. The immune functions of microglia are regulated by a milieu of cytokines including interferon (IFN)-gamma. We here performed a series of experiments to acertain the transcriptional profile of human fetal microglial cells at 1, 6, and 24 h after IFN-gamma treatment. Primary human microglial cells were either untreated o...
Keywords: time-course microglia interferon-gamma macrophages expression profiling by array
102
Rat exposure to RDX (3mg/kg or 18mg/kg; 0, 4, 24, 48 hr)
RDX (Hexahydro-1,3,5-trinitro-1,3,5-triazine) is a synthetic, high-impact, relatively stable explosive that has been in use since WWII. Exposure to RDX can occur either occupationally or through ordnance that lays unexploded on training ranges. The toxicology of RDX is dominated by acute tonic-clonic seizures at high doses, which remit when exposure is removed and internal RDX levels decrease. Sub-chronic studies have revealed few other toxic effects. The objective of this study was to e...
Keywords: time course dose response expression profiling by array
103
Gene expression data of skin from psoriatic patients...
Gene expression has been proposed as an intermediate phenotype that can increase power in complex trait gene-mapping studies. Psoriasis, an immune-mediated, inflammatory and hyperproliferative disease of the skin and joints, provides an ideal model system to evaluate this paradigm, as conclusive evidence demonstrates that psoriasis has a genetic basis and the disease tissue is readily accessible.To better understand the complex nature of processes in psoriasis, we characterize gene expression...
Keywords: disease state analysis expression profiling by array
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