Data Catalog

Time course of bone marrow-derived macrophages simul...

The innate immune system is a two-edged sword; it is absolutely required for host defense against infection, but if left uncontrolled can trigger a plethora of inflammatory diseases. Here we used systems biology approaches to predict and validate a gene regulatory network involving a dynamic interplay between the transcription factors NF-κB, C/EBPδ, and ATF3 that controls inflammatory responses. We mathematically modeled transcriptional regulation of Il6 and Cebpd genes and experimentally v...

Keywords: NCBI GEO macrophages expression profiling by array

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Gene Expression Profiles of RAW264.7 Macrophages Sti...

Background: Toll-like family of receptors recognizes pathogen-associated molecular patterns (PAMPs) from different organisms. TLR4 is the receptor for bacterial lipopolysaccharide (LPS), dsRNA viral mimic poly(I:C) binds to TLR3, and bacterial CpG DNA signals through TLR9. TLR4 signaling is mediated by adaptor molecules Myd88 and TRIF while TLR3 pathway involves only the TRIF adaptor and TLR9 signals solely through Myd88. Methods: To identify genes other than those in TLR pathways th...

Keywords: time course macrophages expression profiling by array

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Human immune cell transcriptome

In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. This comprehensive dataset enabled us to not only establish which genes were expressed in each leukocyte type, but also which genes were expressed in each subset after activation. The used of a comprehensive dataset of gene profiles from all the major human leukocyte subsets enabled a novel and powerful means for identification of genes associated...

Keywords: Cell type comparison macrophages expression profiling by array

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Monocytes of patients with familial hypercholesterol...

Background: Elevated plasma cholesterol promotes the formation of atherosclerotic lesions in which monocyte-derived lipid-laden macrophages are frequently found. To analyze, if circulating monocytes already show increased lipid content and differences in lipoprotein metabolism, we compared monocytes from patients with Familial Hypercholesterolemia (FH) with those from healthy individuals.Methods: Cholesterol and oxidized cholesterol metabolite serum levels of FH and of healthy, gender/age mat...

Keywords: Micro-array analysis cell type comparison, macrophages expression profiling by array

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Similarities and differences in the transcriptome of...

Phenotypic and functional diversity between macrophage subpopulations reflects their plasticity to respond to microenvironmental signals. Apart from detecting differences in expression profiles, the comparison of the transcriptomes of different macrophage populations may also allow the definition of molecular similarities between these subsets.Transcriptome analysis of human Kuppfer cells, alveolar, splenic and atherosclerotic plaque residing macrophages using microarrays, identified 42 genes...

Keywords: NCBI GEO macrophages expression profiling by array

Project website

Expression data from mouse bone marrow macrophages f...

Bone marrow macrophages were cultured from 16 week old apoE-deficient F2 mice from an AKRxDBA/2 intercrossGene expression profiling was performed using Affy 430v2 arrays, and 1967 informative SNP markers were genotyped for each mouseSupplementary file of SNP data attached below from ParAllele 5K mouse linkage panel

Keywords: Genetic-genomic macrophages expression profiling by array

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Gene expression analysis of macrophages from ankylos...

OBJECTIVE: To determine whether macrophages, a type of cell implicated in the pathogenesis of ankylosing spondylitis (AS), exhibit a characteristic gene expression pattern. METHODS: Macrophages were derived from the peripheral blood of 8 AS patients (median disease duration 13 years [range <1-43 years]) and 9 healthy control subjects over 7 days with the use of granulocyte-macrophage colony-stimulating factor. Cells were stimulated for 24 hours with interferon-gamma (IFNgamma; 100 units/ml), ...

Keywords: Disease state analysis with treatment effect macrophages expression profiling by array