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142 projects found
Gene Expression Analysis in Peripheral Blood of Pati...
Peripheral blood cells in patients with rheumatoid arthritis before/after IL-6 inhibitionThe obtained conclusions on the change of each gene expression level before and after MRA medication were as follows.(1) In the case of IL-6, the specific time profile that the expression level dropped at day 21 and increased to some extent at day 42 was observed in 2 of 18 subjects. In other subjects, quite small change or small increase after day 7 was observed.(2) In the case of IL-6R as well as in IL-...
Keywords: NCBI GEO expression profiling by array
81
Rheumatoid arthritis and anti-TNF treatment
In 15 patients with rheumatoid arthritis the baseline expression (t=0) of type I IFN-regulated genes was determined in peripheral blood cells (PAXgene) using cDNA-microarrays and compared to levels one month after (t=1) anti-TNF treatment (infliximab). Therefore, we used 43K cDNA microarrays from the Stanford Functional Genomics Facility (http://microarray.org/sfgf/) printed on aminosilane-coated slides containing ~20.000 unique genes. Only one batch of arrays was used for all experiments. Fi...
Keywords: NCBI GEO expression profiling by array
82
The type of phagocytic receptor modulates gene expre...
The receptors engaged during phagocytic particle uptake determine the signaling events that occur during phagosome formation and maturation. However, pathogens generally have multiple ligands, making it difficult to dissect the roles of individual receptors in these processes. Here, we used latex beads coupled to single ligands, focusing on IgG, mannan, LPS and avidin, and monitored phagocytic uptake rates, phago-lysosomal fusion events, macrophage gene expression and the proteomic compositio...
Keywords: NCBI GEO macrophages expression profiling by array
83
Gene expression analysis of TSC-tubers reveals incre...
Cortical tubers in patients with tuberous sclerosis complex (TSC) are associated with cognitive disability and intractable epilepsy. While these developmental malformations are believed to result from the effects of TSC1 or TSC2 Gene mutations, the molecular mechanisms leading to tuber formation during brain development as well as the onset of seizures remain largely unknown. We used the Affymetrix Gene Chip platform as a genome-wide strategy to define the Gene expression profile of cortical ...
Keywords: NCBI GEO expression profiling by array
84
Expression data from human macrophages
Human CD14 positive monocytes were purified from healthy volunteers’ blood and cultured in vitro for 4, 12, 24, 72 hours. While culturing, macrophages were activated alternatively with interleukin-4 (IL-4 100 ng/ml) or classically with interferon-gamma (IFNg 100 ng/ml)+tumor necrosis factor (TNF 50 ng/ml) or left without activation. Simultaneously, macrophages were also treated with vehicle (DMSO:ethanol) or 1mM synthetic PPARg agonist, Rosiglitazone. We used Affymetrix microarrays (U133Plus ...
Keywords: NCBI GEO macrophages expression profiling by array
85
Expression analysis of dendritic cells, macrophages ...
Expression profiles at different time points during dendritic cell differentiation (induced by specific culture conditions) including monocytes as well as expression profiles between monocytes and completely differentiated cells (macrophages at day7 and dendritic cells at day7, respectively) were compared. Monocyte-derived dendritic cells (DC) were obtained by culturing elutriated monocytes with 20U/ml IL-4, 280U/ml GM-CSF and 10% FCS; monocyte-derived macrophages (MAC) were obtained by cult...
Keywords: NCBI GEO macrophages expression profiling by array
86
Macrophages heterogeneity in human atherosclerotic plaques
This study aims to characterize the transcriptional profile of Granulocyte-macrophage colony-stimulating factor induced macrophages (GM-MØ) and M-CSF macrophages (M-MØ) and to investigate in situ a subset of genes and their products specific to each phenotype in human atherosclerosis plaques
Keywords: NCBI GEO macrophages expression profiling by array
87
Expression analysis of laser-dissected SNpc neurons ...
Genome-wide transcriptome analysis of expression changes in laser-dissected SNpc neurons from Parkinson's disease brain tissue versus control brain tissue.
Keywords: NCBI GEO expression profiling by array
88
