Characteristic DNA methylation profiles in peripheral blood monocytes are associated with inflammatory phenotypes of asthma

Peripheral blood was collected from adults with eosinophilic asthma (EA; n=21), paucigranulocytic asthma (PGA; n=22), neutrophilic asthma (NA; n=9), and healthy controls (n =10). Blood monocytes were isolated using ficoll density gradient and immuno-magnetic cell separation. Bisulfite converted genomic DNA was hybridised to Illumina Infinium Methylation27 arrays, and analysed for differential methylation using R/Bioconductor packages


Primary Purpose
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Description of Cohorts
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Informed Consent Given?
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Multi-center Study?
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Organisms
Homo sapiens
Number of subjects
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Diseases
asthma
Sample Sources
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Type of Samples Collected
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Age Range of Study Participants
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BMI Range of Study Participants
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