Characteristic DNA methylation profiles in peripheral blood monocytes are associated with inflammatory phenotypes of asthma

Peripheral blood was collected from adults with eosinophilic asthma (EA; n=21), paucigranulocytic asthma (PGA; n=22), neutrophilic asthma (NA; n=9), and healthy controls (n =10). Blood monocytes were isolated using ficoll density gradient and immuno-magnetic cell separation. Bisulfite converted genomic DNA was hybridised to Illumina Infinium Methylation27 arrays, and analysed for differential methylation using R/Bioconductor packages